1814个人类7号染色体特异性序列标签位点的集合。
A collection of 1814 human chromosome 7-specific STSs.
作者信息
Bouffard G G, Iyer L M, Idol J R, Braden V V, Cunningham A F, Weintraub L A, Mohr-Tidwell R M, Peluso D C, Fulton R S, Leckie M P, Green E D
出版信息
Genome Res. 1997 Jan;7(1):59-64. doi: 10.1101/gr.7.1.59.
An established goal of the ongoing Human Genome Project is the development and mapping of sequence-tagged sites (STSs) every 100 kb, on average, across all human chromosomes. En route to constructing such a physical map of human chromosome 7, we have generated 1814 chromosome 7-specific STSs. The corresponding PCR assays were designed by the use of DNA sequence determined in our laboratory (79%) or generated elsewhere (21%) and were demonstrated to be suitable for screening yeast artificial chromosome (YAC) libraries. This collection provides the requisite landmarks for constructing a physical map of chromosome 7 at < 100-kb average spacing of STSs.
正在进行的人类基因组计划的既定目标是，平均每隔100 kb在所有人类染色体上开发并绘制序列标签位点（STS）图谱。在构建人类7号染色体物理图谱的过程中，我们已生成了1814个7号染色体特异性STS。相应的聚合酶链反应（PCR）分析是通过使用我们实验室测定的DNA序列（79%）或其他地方生成的序列（21%）设计的，并且已证明适用于筛选酵母人工染色体（YAC）文库。该集合为构建平均STS间距<100 kb的7号染色体物理图谱提供了必要的界标。
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