重组葡萄球菌激酶的结晶及初步X射线分析

Crystallization and preliminary x-ray analysis of recombinant staphylokinase.

作者信息

Rabijns A, Baeyens K, De Bondt H L, De Ranter C

机构信息

Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.

出版信息

Proteins. 1997 Jan;27(1):160-1.

PMID:9037722

Abstract

Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl2, Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions alpha = 60.6 A, b = 43.7 A, c = 54.3 A, and beta = 115.6 degrees. A complete native data set to 1.8 A resolution has been collected using synchrotron radiation.

摘要

通过悬滴气相扩散技术，在含有氯化镁、Tris缓冲液（pH 8.5）和聚乙二醇4000的溶液中生长出了重组葡萄球菌激酶（STAR）的衍射质量晶体。这些晶体属于单斜晶系空间群C2，晶胞参数为α = 60.6 Å，b = 43.7 Å，c = 54.3 Å，β = 115.6°。利用同步辐射收集了分辨率为1.8 Å的完整天然数据集。