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A组火鸡和鸡轮状病毒VP6基因的序列分析

Sequence analysis of the VP6 gene in group A turkey and chicken rotaviruses.

作者信息

Ito H, Minamoto N, Hiraga S, Sugiyama M

机构信息

Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.

出版信息

Virus Res. 1997 Jan;47(1):79-83. doi: 10.1016/s0168-1702(96)01401-3.

DOI:10.1016/s0168-1702(96)01401-3
PMID:9037739
Abstract

cDNAs corresponding to the VP6 gene of the turkey rotavirus strains Ty-1 and Ty-3, and the chicken rotavirus strain Ch-1, were cloned and sequenced. The nucleotide and deduced amino acid sequence homology in the coding region of the VP6 gene in avian rotaviruses ranged from 78.1 to 93.9% and 86.1 to 98.7%, respectively. Both sequences of VP6 from avian rotaviruses exhibited a low degree of sequence homology (67.8-70.7% and 69.8-74.6%, respectively) compared with mammalian rotaviruses. Phylogenetic tree analysis showed that all avian rotaviruses were included in a single cluster and have separated early or from mammalian rotaviruses during evolution. The chicken rotavirus strain Ch-1 was a distant relative of other avian rotaviruses.

摘要

克隆并测序了与火鸡轮状病毒Ty - 1和Ty - 3株以及鸡轮状病毒Ch - 1株的VP6基因相对应的cDNA。禽轮状病毒VP6基因编码区的核苷酸和推导氨基酸序列同源性分别为78.1%至93.9%和86.1%至98.7%。与哺乳动物轮状病毒相比,禽轮状病毒的VP6的两个序列均表现出较低程度的序列同源性(分别为67.8 - 70.7%和69.8 - 74.6%)。系统发育树分析表明,所有禽轮状病毒都包含在一个单一的簇中,并且在进化过程中很早就与哺乳动物轮状病毒分离。鸡轮状病毒Ch - 1株是其他禽轮状病毒的远亲。

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