Martinez A, Blanes A, Ramon y Cajal Junquera S, De Ispizua I, Campo C
J Neurol Sci. 1977 Aug;33(1-2):61-9. doi: 10.1016/0022-510x(77)90182-4.
Experimental allergic neuritis (EAN) was induced in guinea-pigs by intradermal injection of rabbit peripheral nerve emulsified in Freund's adjuvant. Both sciatic nerves were obtained between 12--24 hr after clinical symptoms were evident. Several fascicles from each nerve were isolated for histochemical studies with NADH-diaphorase (NADH) and acid phosphatase (AP) applied to teased nerve fibres. Small pieces were processed for electron microscopy, and a fascicle was teased after staining with osmium tetroxide. In isolated nerve fibres stained with histochemical techniques myelin lesions of segmental character were found closely related to inflammatory cells; Schwann cell cytoplasm in contact with mononuclear cells showing a heavy enzymatic activity (NADH and AP). Under polarized light, the underliying myelin showed focal loss of birefringence. Some electron-microscopic pictures suggested active myelin breakdown by mononuclear cells. The possibility of primary Schwann cell damage by mononuclear cells with subsequent demyelination is discussed.
通过皮内注射弗氏佐剂乳化的兔周围神经，在豚鼠中诱发实验性变应性神经炎（EAN）。在临床症状明显后的12 - 24小时内获取双侧坐骨神经。从每条神经中分离出几个束用于组织化学研究，将烟酰胺腺嘌呤二核苷酸黄递酶（NADH）和酸性磷酸酶（AP）应用于 teased 神经纤维。取小块组织进行电子显微镜检查，并用四氧化锇染色后 teased 一个束。在用组织化学技术染色的分离神经纤维中，发现节段性特征的髓鞘损伤与炎性细胞密切相关；与单核细胞接触的施万细胞胞质显示出强烈的酶活性（NADH和AP）。在偏振光下，下层髓鞘显示出双折射的局灶性丧失。一些电子显微镜照片提示单核细胞对髓鞘有活跃的破坏作用。讨论了单核细胞对施万细胞原发性损伤并随后导致脱髓鞘的可能性。
