Ochi K, Ohashi T, Kato I, Eggermont J J
Department of Otolaryngology, St. Marianna University School of Medicine, Kanagawa.
Nihon Jibiinkoka Gakkai Kaiho. 1997 Jan;100(1):51-8. doi: 10.3950/jibiinkoka.100.51.
The effect of salicylate and quinine on the spontaneous firing rate in the cat primary auditory cortex was investigated in 13 healthy cats. Spontaneous firing rates were calculated for each single unit. A dose of 200 mg of sodium salicylate per kg was administered intraperitoneally to six cats, and the findings from the same single unit were recorded prior to application and continuously up to, on average, 6 hours after application. A dose of 100 or 200 mg of quinine hydrochloride per kg was administered intramuscularly to seven cats, and the findings from the same single unit were recorded in the same manner as for the cats treated with sodium salicylate. Twenty one single units in salicylate-treated cats and 29 single units in quinine-treated cats were evaluated. All animals treated with salicylate showed a 20-30 dB threshold shift about 2 hours after application and showed no recovery during the course of the investigation. All animals treated with quinine showed a 10-40 dB threshold shift about 1 hour after application and recovered during the course of the investigation. There was no consistent difference in overall spontaneous firing rate before and after application in either salicylate-treated cats or quinine treated cats. In order to investigate a potential different effect on units with different spontaneous firing rates, we divided the cats into two groups, a high-firing rate group (pre-application firing rate > 1 spike/s) and a low firing rate group (pre-application firing rate < 1 spike/s). A significant decrease in the high-firing rate group (p < 0.05) and a significant increase in the low firing rate (p < 0.01) were observed in salicylate-treated cats. The same tendencies were observed in quinine-treated cats, but only the difference in the low firing rate group was significant (p < 0.05). The difference in the high-firing rate group was close to the significant level (p = 0.055). These changes in spontaneous firing rates in cat primary auditory cortex may be related to the generation of tinnitus.
在13只健康猫中研究了水杨酸盐和奎宁对猫初级听觉皮层自发放电率的影响。计算每个单个神经元的自发放电率。给6只猫腹腔注射每千克200毫克的水杨酸钠，在注射前记录同一单个神经元的情况，并持续记录直至注射后平均6小时。给7只猫肌肉注射每千克100或200毫克的盐酸奎宁，以与水杨酸钠处理的猫相同的方式记录同一单个神经元的情况。对水杨酸盐处理的猫中的21个单个神经元和奎宁处理的猫中的29个单个神经元进行了评估。所有接受水杨酸盐治疗的动物在注射后约2小时出现20 - 30分贝的阈值偏移，并且在研究过程中未恢复。所有接受奎宁治疗的动物在注射后约1小时出现10 - 40分贝的阈值偏移，并在研究过程中恢复。在水杨酸盐处理的猫或奎宁处理的猫中，注射前后的总体自发放电率没有一致的差异。为了研究对具有不同自发放电率的神经元的潜在不同影响，我们将猫分为两组，高放电率组（注射前放电率>1个脉冲/秒）和低放电率组（注射前放电率<1个脉冲/秒）。在水杨酸盐处理的猫中，高放电率组有显著下降（p<0.05），低放电率组有显著增加（p<0.01）。在奎宁处理的猫中也观察到相同的趋势，但只有低放电率组的差异显著（p<0.05）。高放电率组的差异接近显著水平（p = 0.055）。猫初级听觉皮层自发放电率的这些变化可能与耳鸣的产生有关。
