牙龈卟啉单胞菌W83膜相关蛋白酶的可诱导表达
Inducible expression of a Porphyromonas gingivalis W83 membrane-associated protease.
作者信息
Park Y, Lu B, Mazur C, McBride B C
机构信息
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
出版信息
Infect Immun. 1997 Mar;65(3):1101-4. doi: 10.1128/IAI.65.3.1101-1104.1997.
Abstract
The Tpr protease of Porphyromonas gingivalis W83 is a membrane-associated enzyme capable of hydrolyzing a chromogenic bacterial collagenase substrate. An isogenic mutant lacking a functional tpr gene had a greatly reduced ability to hydrolyze the collagenase substrate. Activity was restored to the tpr mutant by introducing a shuttle plasmid containing the tpr gene. Expression of the gene is induced by nutrient limitation, as shown by enzymatic and Northern analyses.
摘要
牙龈卟啉单胞菌W83的Tpr蛋白酶是一种与膜相关的酶，能够水解一种产色细菌胶原酶底物。缺乏功能性tpr基因的同基因突变体水解胶原酶底物的能力大大降低。通过导入含有tpr基因的穿梭质粒，tpr突变体的活性得以恢复。如酶分析和Northern分析所示，该基因的表达受营养限制诱导。
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