Roşianu A, Tudose N, Potencz E
Dept. of Morphopatology, University of Medicine and Pharmacy, Timişoara.
Rom J Morphol Embryol. 1996 Jan-Jun;42(1-2):63-71.
A certain proportion of breast cancers contain hormone receptors--specific proteins with high affinity and capacity of selective tie of these hormones. There were selected 30 patients presenting breast cancer, treated at the surgical departments from the County Hospital of Timişoara between 1993 and 1995. The identification of hormone receptors was done on 10% formalin-fixed and paraffin-embedded tissue specimens. As anti-hormone receptors, monoclonal and polyclonal antibodies were used. The immunohistochemical identification of positive to hormone receptors cancer cells was obtained, by nucleus staining. Immunohistochemical assay of the accumulation of p53 protein seems to be an efficient means of selecting breast neoplasms. p53 immunostaining is positive at the level of malignant cells' nuclei and negative for normal cells. From the 30 breast carcinomas, 19 (63.3%) were ER+ and 15 (49.9%) were PR+, resulting an increase of the frequency of ER+ tumors together with the age of the patients; 9 cases (30%) expressed p53 protein, harmonizing with the data in the literature.
一定比例的乳腺癌含有激素受体，即对这些激素具有高亲和力和选择性结合能力的特定蛋白质。选取了1993年至1995年间在蒂米什瓦拉县医院外科接受治疗的30例乳腺癌患者。激素受体的鉴定在10%中性福尔马林固定、石蜡包埋的组织标本上进行。作为抗激素受体，使用了单克隆抗体和多克隆抗体。通过细胞核染色，对激素受体呈阳性的癌细胞进行免疫组织化学鉴定。p53蛋白积累的免疫组织化学检测似乎是筛选乳腺肿瘤的有效手段。p53免疫染色在恶性细胞核水平呈阳性，而在正常细胞中呈阴性。在这30例乳腺癌中，19例（63.3%）雌激素受体（ER）呈阳性，15例（49.9%）孕激素受体（PR）呈阳性，ER阳性肿瘤的发生率随患者年龄增加而升高；9例（30%）表达p53蛋白，与文献数据相符。
