Hosken D J, O'Shea J E, Blackberry M A
Department of Zoology, University of Western Australia, Nedlands, Australia.
J Reprod Fertil. 1996 Nov;108(2):171-7. doi: 10.1530/jrf.0.1080171.
The fertility and viability of spermatozoa stored by male and female Gould's wattled bats, Chalinolobus gouldii, was investigated in a captive colony of ten bats (three males and seven females). Bats were housed in outdoor flight cages. Plasma progesterone concentrations, measured using double antibody radioimmunoassay, isolation experiments plus sperm motility and sperm membrane stability tests were used to evaluate the viability and fertility of stored spermatozoa. Mean plasma progesterone concentrations were lowest during midwinter (< 0.5 ng ml-1) with a 20-fold increase recorded in late winter to early spring. During pregnancy, plasma progesterone concentrations increased to about 13 ng ml-1 and returned to basal values soon after parturition. The results of the plasma progesterone assays and the isolation experiments indicate that female C. gouldii can store fertile spermatozoa for at least 33 days. The investigation of spermatozoa stored by male C. gouldii revealed that 6-7 months after peak spermatogenesis about 60% of the stored spermatozoa were motile and more than 60% had stable membranes, indicating that the spermatozoa stored by males were viable and likely to be fertile. The results of this study clearly indicate that both male and female C. gouldii are capable of storing fertile spermatozoa for prolonged periods.
在一个由十只古尔德氏垂耳蝠（雄性三只，雌性七只）组成的圈养群体中，对雄性和雌性古尔德氏垂耳蝠储存精子的生育能力和活力进行了研究。蝙蝠被饲养在室外飞行笼中。使用双抗体放射免疫分析法测量血浆孕酮浓度，并通过隔离实验以及精子活力和精子膜稳定性测试来评估储存精子的活力和生育能力。冬季中期血浆孕酮平均浓度最低（<0.5纳克/毫升），冬末至早春期间记录到浓度增加了20倍。在怀孕期间，血浆孕酮浓度增加到约13纳克/毫升，分娩后不久又恢复到基础值。血浆孕酮测定和隔离实验的结果表明，雌性古尔德氏垂耳蝠可以将可育精子储存至少33天。对雄性古尔德氏垂耳蝠储存精子的研究表明，精子发生高峰期6 - 7个月后，约60%的储存精子具有活力，超过60%的精子膜稳定，这表明雄性储存的精子具有活力且可能具有生育能力。这项研究的结果清楚地表明，雄性和雌性古尔德氏垂耳蝠都能够长时间储存可育精子。
