Hynes A C, Sreenan J M, Kane M T
Department of Physiology, University College, Galway, Ireland.
J Reprod Fertil. 1996 Nov;108(2):193-7. doi: 10.1530/jrf.0.1080193.
A granulosa cell-inhibitory factor (GCIF) of low molecular mass from bovine follicular fluid inhibits the proliferation of bovine granulosa cells in vitro and the growth of large follicles in rats in vivo. The present study examined the ability of GCIF to modulate the effects of FSH, epidermal growth factor (EGF), insulin-like growth factor I (IGF-I) and androstenedione on the proliferation of bovine granulosa cells and on aromatase activity in vitro. Granulosa cell proliferation was assayed by counting haemocytometric cells and by measuring the incorporation of [3H]thymidine into acid-precipitable material. Assay of aromatase activity was based on the conversion of [3H]androstenedione to [3H]H2O. FSH, androstenedione, EGF and IGF-I all stimulated (P < 0.01) granulosa cell proliferation; however, the addition of GCIF reduced (P < 0.01) cell proliferation in their presence. In the case of EGF, the addition of GCIF almost abolished the stimulatory response. FSH and IGF-I, but not EGF, stimulated (P < 0.01) aromatase activity of granulosa cells. The stimulatory effect of IGF-I was decreased by GCIF. The inhibitory effects of GCIF indicate that it may play a significant role in regulating the effects of intraovarian growth factors on granulosa cells and the growth of follicles.
来自牛卵泡液的一种低分子量颗粒细胞抑制因子（GCIF）在体外可抑制牛颗粒细胞的增殖，并在体内抑制大鼠大卵泡的生长。本研究检测了GCIF调节促卵泡激素（FSH）、表皮生长因子（EGF）、胰岛素样生长因子I（IGF-I）和雄烯二酮对牛颗粒细胞增殖及体外芳香化酶活性影响的能力。通过血细胞计数法计数细胞以及测量[3H]胸腺嘧啶核苷掺入酸沉淀物质中的量来检测颗粒细胞增殖。芳香化酶活性测定基于[3H]雄烯二酮向[3H]H2O的转化。FSH、雄烯二酮、EGF和IGF-I均刺激（P<0.01）颗粒细胞增殖；然而，加入GCIF会降低（P<0.01）它们存在时的细胞增殖。就EGF而言，加入GCIF几乎消除了刺激反应。FSH和IGF-I刺激（P<0.01）颗粒细胞的芳香化酶活性，而EGF则无此作用。GCIF降低了IGF-I的刺激作用。GCIF的抑制作用表明，它可能在调节卵巢内生长因子对颗粒细胞的作用及卵泡生长方面发挥重要作用。
