Boulton M I, McGrath T J, Goode J A, Broad K D, Gilbert C L
Department of Neurobiology, Babraham Institute, Cambridge, UK.
J Reprod Fertil. 1996 Nov;108(2):219-27. doi: 10.1530/jrf.0.1080219.
The aim of this study was to show that the pig uterus synthesizes oxytocin. Uteri were obtained from 2-7 pigs at regular intervals during the oestrous cycle, throughout pregnancy, at parturition and in lactational anoestrus. Localization of mRNA encoding oxytocin was by in situ hybridization and oxytocin concentrations were measured by radioimmunoassay. As reproductive status changed, mRNA encoding oxytocin varied significantly (P < 0.05). Uterine tissue type was a significant factor in determining synthesis of mRNA encoding oxytocin (P < 0.001). In luminal epithelia, concentrations of mRNA encoding oxytocin were greater at oestrus than during day 14 of the luteal phase (P < 0.01) or at any stage of pregnancy (P < 0.05), with concentrations minimal at parturition. This trend was also exhibited in uterine circular muscle. In longitudinal muscle, concentrations of mRNA encoding oxytocin were lower during late pregnancy than at oestrus (P < 0.05) or during the luteal phase (P < 0.05). Concentrations were minimal at parturition. The oxytocin content in endometrial and myometrial tissue was positively correlated across reproductive status (P < 0.02, r = 0.402, n = 35). These data are the first indication that the uterine endometrium and musculature of the pig express mRNA encoding oxytocin. The luminal epithelium of animals at oestrus was particularly rich in mRNA encoding oxytocin, whilst late pregnant and parturient animals did not show a rise in mRNA encoding oxytocin. Local uterine synthesis of oxytocin may therefore be more important in control of the oestrous cycle than in pregnancy or at parturition in pigs.
本研究的目的是证明猪子宫能合成催产素。在发情周期的不同阶段、整个妊娠期、分娩时和泌乳期乏情期，每隔一定时间从2 - 7头猪获取子宫。通过原位杂交确定编码催产素的mRNA的定位，并用放射免疫分析法测定催产素浓度。随着生殖状态的变化，编码催产素的mRNA有显著差异（P < 0.05）。子宫组织类型是决定编码催产素的mRNA合成的一个重要因素（P < 0.001）。在腔上皮中，发情期编码催产素的mRNA浓度高于黄体期第14天（P < 0.01）或妊娠任何阶段（P < 0.05），分娩时浓度最低。子宫环肌也呈现这种趋势。在纵肌中，妊娠后期编码催产素的mRNA浓度低于发情期（P < 0.05）或黄体期（P < 0.05），分娩时浓度最低。子宫内膜和肌层组织中的催产素含量在不同生殖状态下呈正相关（P < 0.02，r = 0.402，n = 35）。这些数据首次表明猪的子宫内膜和肌肉组织表达编码催产素的mRNA。发情期动物的腔上皮中编码催产素的mRNA特别丰富，而妊娠后期和分娩动物编码催产素的mRNA并未升高。因此，在猪中，局部子宫合成催产素在发情周期的调控中可能比在妊娠或分娩时更为重要。
