Rowe A, Farrell A M, Bunker C B
Skin Treatment and Research Trust Laboratory, Charing Cross and Westminster Medical School, Chelsea and Westminster Hospital, London, UK.
Br J Dermatol. 1997 Jan;136(1):18-23.
We have used immunohistochemistry to localize the expression of the constitutive endothelial and inducible forms of the enzyme nitric oxide synthase (NOS) in skin from involved and uninvolved sites in patients with atopic dermatitis (AD) and allergic contact dermatitis (CD). Endothelial NOS (eNOS) immunoreactivity was localized to vascular endothelium in the dermis of both involved and uninvolved skin from all patients. Inducible NOS (iNOS) immunoreactivity was found to be closely associated with the upper dermal microvasculature in all the involved AD biopsies, but only in two of 10 uninvolved AD biopsies. CD biopsies were taken from 10 positive skin patch test sites and iNOS immunoreactivity was detected in all of these. iNOS immunoreactivity was detected in only one of the negative patch test biopsies. Both the extent and intensity of iNOS immunoreactivity was lower in CD than in AD skin lesions. The presence of eNOS in the skin is necessary for constitutive NO-mediated dilatation of the dermal vasculature. Induction of iNOS in the dermal endothelium and in perivascular inflammatory cells may be significant with respect to the roles of NO in both the vasodilatory component of the inflammatory response and in the modulation of immune responses in the skin.
我们运用免疫组织化学方法，对特应性皮炎（AD）和过敏性接触性皮炎（CD）患者受累及未受累部位皮肤中组成型内皮型和诱导型一氧化氮合酶（NOS）的表达进行定位。在所有患者的受累及未受累皮肤的真皮层中，内皮型一氧化氮合酶（eNOS）免疫反应性定位于血管内皮。在所有受累的AD活检组织中，诱导型一氧化氮合酶（iNOS）免疫反应性与真皮上部微血管密切相关，但在10份未受累的AD活检组织中仅两份如此。CD活检组织取自10个阳性皮肤斑贴试验部位，所有这些部位均检测到iNOS免疫反应性。在阴性斑贴试验活检组织中仅一份检测到iNOS免疫反应性。CD中iNOS免疫反应性的范围和强度均低于AD皮肤病变。皮肤中eNOS的存在对于组成型NO介导的真皮血管舒张是必要的。真皮内皮和血管周围炎症细胞中iNOS的诱导对于NO在炎症反应的血管舒张成分以及皮肤免疫反应调节中的作用可能具有重要意义。
