Sasaki T
Rice Genome Research Program (RGP), Tsukuba, Japan.
Symp Soc Exp Biol. 1996;50:11-5.
Large-scale rice cDNA analysis has produced a huge amount of nucleotide sequence information for expressed genes in rice. The genes of cDNA clones putatively identified by similarity search were originally found in many different organisms. However, genes identified at a higher confidence level were found in plants, especially in monocots. This means the sequence information produced in random cloning of rice cDNA is useful for the study of other Gramineae. Further, assigned gene names of cDNAs mapped on linkage group 6 were grouped by their original species. The functions of gene products for 51% of mapped cDNAs were assigned and 67% of them were known in plants. The map information obtained by linking the position of a cDNA locus and its assigned gene function is indispensable for elucidating collinearity of genes among plant genomes.
大规模水稻cDNA分析已产生了大量水稻中表达基因的核苷酸序列信息。通过相似性搜索推测鉴定出的cDNA克隆基因最初在许多不同生物中被发现。然而，在植物中，尤其是单子叶植物中发现了置信度更高的基因。这意味着水稻cDNA随机克隆产生的序列信息对其他禾本科植物的研究有用。此外，定位在第6连锁群上的cDNA所指定的基因名称按其原始物种进行了分组。51%的定位cDNA的基因产物功能已被指定，其中67%在植物中是已知的。通过将cDNA位点的位置与其指定的基因功能联系起来获得的图谱信息对于阐明植物基因组间基因的共线性是必不可少的。
