Kakoi H, Anniko M, Kinnefors A, Rask-Andersen H
Department of Otorhinolaryngology, University Hospital (Akademiska sjukhuset), Uppsala, Sweden.
Acta Otolaryngol. 1997 Jan;117(1):100-8. doi: 10.3109/00016489709117999.
A location of proliferating cells was investigated in eight human normal tympanic membranes (TMs) and external auditory canals (EACs) by an immunohistochemical method using two different types of antibodies for nuclear antigens in proliferating cells: anti-PCNA monoclonal antibody, and anti-Ki-67 polyclonal antibody. Four specimens prepared for cryostat sections were immunostained by both antibodies. Another four were fixed in 4% formaldehyde solution, embedded in paraffin wax and were reacted only with anti-PCNA antibodies. The expression pattern of Ki-67 was basically the same as of PCNA. In the pars tensa (PT), immunoreactivities were expressed in the nuclei of basal layer cells and cells just overlying the basal layer of epidermis both in the handle of the malleus (HM) and annular regions. In the intermediate region of the PT, no immunoreactivity was found basically, apart from a few labelled cells observed in the upper-third of the superior quadrant. In the pars flaccida (PF) and in both the osseous and cartilaginous regions of the EAC, positive cells were also situated in the basal layer and the deeper aspect of the suprabasal layers without any specific distributing pattern. It was certified that the generation centre of epidermal cells (keratinocytes) in the PT was located in both the HM and annular regions, and that stem cells in the PF and the EAC were uniformly scattered in the basal layer and the deeper aspect of the spinous layer. According to these findings, the migratory patterns of auditory epidermal cells in the human TM and EAC were discussed.
通过免疫组织化学方法，使用两种针对增殖细胞核抗原的不同类型抗体，即抗增殖细胞核抗原（PCNA）单克隆抗体和抗Ki-67多克隆抗体，对8例正常人类鼓膜（TM）和外耳道（EAC）中增殖细胞的位置进行了研究。为冷冻切片制备的4个标本用两种抗体进行免疫染色。另外4个标本用4%甲醛溶液固定，石蜡包埋，仅与抗PCNA抗体反应。Ki-67的表达模式与PCNA基本相同。在紧张部（PT），锤骨柄（HM）和环形区域的表皮基底层细胞以及基底层上方的细胞的细胞核中均有免疫反应性表达。在PT的中间区域，除了在上象限上三分之一处观察到少数标记细胞外，基本未发现免疫反应性。在松弛部（PF）以及EAC的骨部和软骨部区域，阳性细胞也位于基底层和基底上层的较深层面，没有任何特定的分布模式。证实了PT中表皮细胞（角质形成细胞）的生发中心位于HM和环形区域，PF和EAC中的干细胞均匀地分散在基底层和棘层的较深层面。根据这些发现，讨论了人类TM和EAC中听觉表皮细胞的迁移模式。
