Toyoda A, Sakai T, Sugiyama Y, Kusuda J, Hashimoto K, Maeda H
Department of Bioengineering, Faculty of Engineering, Soka University, Tokyo, Japan.
DNA Res. 1996 Oct 31;3(5):337-40. doi: 10.1093/dnares/3.5.337.
We screened potential promoter regions from NotI-linking cosmid clones mapped on human chromosome Xq28 region with our constructed trapping vector and isolated six fragments containing transcription activity. Using one of the obtained fragments as a probe, a novel gene was isolated by screening a human skeletal muscle cDNA library. The isolated cDNA, termed HXC-26, contained an open reading frame of 975 nucleotides encoding 325 amino acids (38,848 Da). The HXC-26 gene was composed of 13 exons that span approximately 8 kb. Several potential GC boxes were found in the putative promoter region, but no typical TATA box. The HXC-26 gene associated with a CpG island was located adjacent to the rab GDP dissociation inhibitor (GDI) gene.
