Alvarez-Pellitero P, Palenzuela O, Sitjà-Bobadilla A
Instituto de Acuicultura Torre de Sal, Castellón, Spain.
Parasitol Res. 1997;83(2):126-36. doi: 10.1007/s004360050222.
The ultrastructure and cytochemistry of merogonial, gamogonial, and early sporogonial stages of Eimeria sparis in the intestine of Sparus aurata were studied. Mature stages showed the typical pellicle, which was lost in some transitional stages. An apparent unit membrane was seen in some immature stages. A parasitophorous vacuole, sometimes with membrane vesicular invaginations, was usually observed. We propose that the stages located over the epithelium and the so-called epicellular stages be termed supraepithelial stages. Endomerogony was observed in intraepithelial and supraepithelial positions. Intraepithelial stages apparently starting ectomerogony were also detected. Electron-dense granules similar to wall-forming-like bodies of types 1 and 2 were observed. Microgametes exhibited two flagella. Cytochemistry study revealed scarce polysaccharides, if any, in merogonial stages and in microgamonts. The occurrence of polysaccharides and amylopectin granules increased progressively in macrogamonts, macrogametes, zygotes, and early oocysts. Lipidic droplets were scarce of absent in merogonial stages and abundant in maturing macrogamonts. Some glycoproteins were demonstrated in certain merogonial stages.
对金头鲷肠道中 Sparus aurata艾美球虫的裂殖体、配子体和早期孢子体阶段的超微结构和细胞化学进行了研究。成熟阶段显示出典型的表膜，在一些过渡阶段会消失。在一些未成熟阶段可见明显的单位膜。通常观察到一个寄生泡，有时带有膜泡内陷。我们建议将位于上皮细胞上方的阶段和所谓的上皮细胞外阶段称为上皮细胞上阶段。在内皮细胞内和上皮细胞上位置观察到内发育。还检测到明显开始外发育的内皮细胞内阶段。观察到类似于1型和2型壁形成样体的电子致密颗粒。小配子有两根鞭毛。细胞化学研究表明，裂殖体阶段和小配子体中几乎没有多糖（如果有的话）。多糖和支链淀粉颗粒在大配子体、大配子、合子和早期卵囊中逐渐增加。脂质滴在裂殖体阶段很少或不存在，在成熟的大配子体中丰富。在某些裂殖体阶段证实了一些糖蛋白的存在。
