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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Krusekopf S, Kleeberg U, Hildebrandt A G, Ruckpaul K

Max-Delbrück-Centre for Molecular Medicine, Berlin, Germany.

Xenobiotica. 1997 Jan;27(1):1-9. doi: 10.1080/004982597240721.

Induction of endogenous cytochrome P4501A1 (CYP1A1) by benzimidazole derivatives has been investigated in the human hepatoma cell line HepG2. 2. By Northern and Western blot analysis, omeprazole has been shown to be a more potent inducer of CYP1A1 than both lansoprazole and E3810, whereas pantoprazole did not induce CYP1A1. Similar results were obtained for the CYP1A1 enzyme-specific deethylation of 7-ethoxyresorufin. 3. The induction of CYP1A1 in the permanent cell line HepG2 corresponds to results observed in human hepatocytes in primary culture. 4. The results provide experimental evidence that HepG2 cells can be used as an appropriate tool to examine inducing effects of drugs on the expression of CYP1A1.

已在人肝癌细胞系HepG2中研究了苯并咪唑衍生物对内源性细胞色素P4501A1（CYP1A1）的诱导作用。2. 通过Northern和Western印迹分析表明，奥美拉唑是比兰索拉唑和E3810更强效的CYP1A1诱导剂，而泮托拉唑不诱导CYP1A1。对于7-乙氧基试卤灵的CYP1A1酶特异性脱乙基反应也得到了类似结果。3. 在永久性细胞系HepG2中CYP1A1的诱导作用与在原代培养的人肝细胞中观察到的结果一致。4. 这些结果提供了实验证据，表明HepG2细胞可作为检测药物对CYP1A1表达诱导作用的合适工具。

Krusekopf S, Kleeberg U, Hildebrandt A G, Ruckpaul K

Max-Delbrück-Centre for Molecular Medicine, Berlin, Germany.

Xenobiotica. 1997 Jan;27(1):1-9. doi: 10.1080/004982597240721.

Induction of endogenous cytochrome P4501A1 (CYP1A1) by benzimidazole derivatives has been investigated in the human hepatoma cell line HepG2. 2. By Northern and Western blot analysis, omeprazole has been shown to be a more potent inducer of CYP1A1 than both lansoprazole and E3810, whereas pantoprazole did not induce CYP1A1. Similar results were obtained for the CYP1A1 enzyme-specific deethylation of 7-ethoxyresorufin. 3. The induction of CYP1A1 in the permanent cell line HepG2 corresponds to results observed in human hepatocytes in primary culture. 4. The results provide experimental evidence that HepG2 cells can be used as an appropriate tool to examine inducing effects of drugs on the expression of CYP1A1.

已在人肝癌细胞系HepG2中研究了苯并咪唑衍生物对内源性细胞色素P4501A1（CYP1A1）的诱导作用。2. 通过Northern和Western印迹分析表明，奥美拉唑是比兰索拉唑和E3810更强效的CYP1A1诱导剂，而泮托拉唑不诱导CYP1A1。对于7-乙氧基试卤灵的CYP1A1酶特异性脱乙基反应也得到了类似结果。3. 在永久性细胞系HepG2中CYP1A1的诱导作用与在原代培养的人肝细胞中观察到的结果一致。4. 这些结果提供了实验证据，表明HepG2细胞可作为检测药物对CYP1A1表达诱导作用的合适工具。