Chen T R
American Type Culture Collection, Rockville, MD 20852, USA.
Anticancer Res. 1996 Nov-Dec;16(6B):3377-85.
The karyotypic characteristics and clonal composition of human normal diploid MRC-5 (46,XY) and tumorigenic SK-UT-1 (45,XX,-13) and SK-UT-IB (46,XX) cell lines were studied. In MRC-5, 45, XX, -6; 45,X; 46, XY, t(7;14) (p11:q24) and 47, XY, M1 clones were identified. Other karyotypic characteristics included 2.4% polyploidy, 4.8% breaks/gaps, 4.1% structural anomalies, 1.4% hyperdiploidy, and 5.4% hypodiploidy. In SK-UT-1, 45, XX, -13, and clones with t(18;22) (q22.2;q11.23) and 46, XX, 4p+ occurred at 56.6%, 39.3%, and 4.1%, respectively. The t(18;22) karyotypes differed by either paired N13, one N13 plus one i(13q), or one i(13q). The significance of single chromosome amplification relating to the outgrowth in one culture by the t(18;22) clone is discussed. Structural aberrations occurred at high frequencies in N3, N6, and N7, and breaks from these chromosomes were clustered at 3p12, 3q21, 6q21, 6q23, and 7q11.2. The highly malignant SK-UT-IB had the stable karyotype and one 46X, f clone only. Clearly, the cell population was basically mosaic, containing more than one co-existing clone plus the polyploidy of each clone.
对人正常二倍体MRC-5（46,XY）、致瘤性SK-UT-1（45,XX,-13）和SK-UT-1B（46,XX）细胞系的核型特征和克隆组成进行了研究。在MRC-5中，鉴定出45,XX,-6；45,X；46,XY,t(7;14)(p11:q24)和47,XY,M1克隆。其他核型特征包括2.4%的多倍体、4.8%的断裂/间隙、4.1%的结构异常、1.4%的超二倍体和5.4%的亚二倍体。在SK-UT-1中，45,XX,-13以及t(18;22)(q22.2;q11.23)和46,XX,4p+的克隆分别占56.6%、39.3%和4.1%。t(18;22)核型的差异在于配对的N13、一个N13加一个i(13q)或一个i(13q)。讨论了与t(18;22)克隆在一种培养物中的生长相关的单染色体扩增的意义。结构畸变在N3、N6和N7中高频出现，这些染色体的断裂聚集在3p12、3q21、6q21、6q23和7q11.2。高度恶性的SK-UT-1B具有稳定的核型，仅一个46X,f克隆。显然，细胞群体基本上是嵌合体，包含不止一个共存克隆以及每个克隆的多倍体。
