Sakakibara Y, Katafuchi J, Takami Y, Nakayama T, Suiko M, Nakajima H, Liu M C
Department of Biochemistry, University of Texas Health Center at Tyler 75710, USA.
Biochim Biophys Acta. 1997 Feb 4;1355(2):102-6. doi: 10.1016/s0167-4889(96)00166-8.
Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.
人类单胺（M）型酚磺基转移酶（PST）通过聚合酶链反应（PCR）克隆，并在COS-7细胞中瞬时表达。已证明重组酶不仅对多巴胺具有先前报道的磺基转移酶活性，还具有新的锰依赖性多巴/酪氨酸磺基转移酶活性。这些结果暗示了人类M型PST在多巴和酪氨酸稳态调节中的新功能作用。
