Ohlson S, Bergström M, Påhlsson P, Lundblad A
University of Kalmar, Department of Natural Sciences, Sweden.
J Chromatogr A. 1997 Jan 17;758(2):199-208. doi: 10.1016/s0021-9673(96)00733-9.
Weak monoclonal antibodies were used as ligands in a high-performance liquid affinity chromatography system. Isocratic weak affinity chromatography was achieved when similar carbohydrate antigens were separated according to their weak binding to the immobilized monoclonal antibody. These chromatographic systems were studied in detail in terms of affinity, specificity and efficiency. The influence of the physico-chemical factors of temperature, pH, ionic strength and organic solvents was also evaluated. The issue of specificity was specially considered, as non-specific interactions are prevalent and usually of a weak affinity nature. This study clearly demonstrates the potential to use weak affinity biological interactions as the basis of chromatographic analysis and separation.
在高效液相亲和色谱系统中，弱单克隆抗体被用作配体。当相似的碳水化合物抗原根据它们与固定化单克隆抗体的弱结合进行分离时，实现了等度弱亲和色谱。从亲和力、特异性和效率方面对这些色谱系统进行了详细研究。还评估了温度、pH、离子强度和有机溶剂等物理化学因素的影响。由于非特异性相互作用普遍存在且通常具有弱亲和性质，因此特别考虑了特异性问题。这项研究清楚地证明了利用弱亲和生物相互作用作为色谱分析和分离基础的潜力。
