Alonso G, Guillemain I, Dumoulin A, Privat A, Patey G
INSERM U336, ENSCM, 8 Rue de l'Ecole Normale, F-34053 Montpellier Cédex 01, France.
Cell Tissue Res. 1997 Apr;288(1):59-68. doi: 10.1007/s004410050792.
A polyclonal antibody raised against a peptide corresponding to the (2-19) amino-terminal sequence of the Bcl-xL/S protein was used to localize Bcl-x immunostaining in the central nervous system of rats at various postnatal ages. Whereas Bcl-x immunostaining was present in virtually all neurons of young animals (4 days postnatal), this staining became progressively restricted during the course of postnatal development. In adults, Bcl-x immunostaining was particularly strong in certain neurons present in a few hypothalamic nuclei, such as the supraoptic or the arcuate nuclei. Moderate staining was observed in some discrete brain regions, such as the olfactory bulb, the hippocampus, some catecholaminergic nuclei of the brainstem, and the cerebellum. Strong Bcl-x immunostaining was also exhibited in axon-like fibers located in the pyriform cortex, the median eminence, the dorsal medulla oblongata, and spinal cord. Bcl-x immunostaining was also present in astrocytes scattered throughout the white matter in the brain and the spinal cord, but was absent from those located in gray matter. Staining was particularly strongly expressed in reactive astrocytes densely packed along the borders of a central lesion or surrounding them, and in a large number of reactive astrocytes detected at a distance from the lesion. Our data suggest that, in addition to the possible stimulating effects on cell survival generally ascribed to Bcl-x, its maintained expression throughout adulthood or its re-expression following injury characterizes those neuronal or non-neuronal cells of the adult central nervous system that synthesize a range of molecules enabling them to adapt rapidly and successfully to a changing environment.
一种针对与Bcl-xL/S蛋白（2-19）氨基末端序列相对应的肽段产生的多克隆抗体，用于在不同出生后年龄的大鼠中枢神经系统中定位Bcl-x免疫染色。在幼龄动物（出生后4天）的几乎所有神经元中都存在Bcl-x免疫染色，但在出生后发育过程中，这种染色逐渐受到限制。在成年大鼠中，Bcl-x免疫染色在少数下丘脑核（如视上核或弓状核）中的某些神经元中特别强烈。在一些离散的脑区，如嗅球、海马体、脑干的一些儿茶酚胺能核团和小脑中观察到中等程度的染色。在位于梨状皮质、正中隆起、延髓背侧和脊髓的轴突样纤维中也表现出强烈的Bcl-x免疫染色。Bcl-x免疫染色也存在于散布在脑和脊髓白质中的星形胶质细胞中，但不存在于灰质中的星形胶质细胞中。染色在沿着中央病变边界密集排列或围绕病变的反应性星形胶质细胞中特别强烈地表达，并且在远离病变处检测到的大量反应性星形胶质细胞中也有表达。我们的数据表明，除了通常归因于Bcl-x对细胞存活的可能刺激作用外，其在成年期的持续表达或损伤后的重新表达是成年中枢神经系统中那些合成一系列分子使其能够快速成功适应变化环境的神经元或非神经元细胞的特征。
