Kieffer F, Simon-Plas F, Maume B F, Blein J P
Unité associée INRA/Université de Bourgogne 692, Laboratoire de Phytoipharmacie et de Biochimie des Interactions Cellulaire, Dijon, France.
FEBS Lett. 1997 Feb 17;403(2):149-53. doi: 10.1016/s0014-5793(97)00038-0.
Suspension-cultured cells of Nicotiana tabacum generated active oxygen species (AOS) when they were treated with the proteinaceous elicitor, cryptogein. This response was blocked by diphenylene iodonium, an inhibitor of the neutrophil NADPH oxidase. When microsomal extracts of tobacco cells were probed with an antibody directed against the human small G protein Rac2, two immunoreactive proteins were detected at 18.5 and 20.5 kDa. The same experiment performed with cytosolic extracts of tobacco cells led to the observation of a strong immunoreactive protein at 21.5 kDa only in the cryptogein-treated cells. The appearance of this cytosolic protein was related to the production of AOS by the elicited cells. These results provide evidence for the possible involvement of small G proteins, homologous to the neutrophil Rac2 protein, in the regulation of the elicitor-induced oxidative burst in plant.
烟草悬浮培养细胞在用蛋白质激发子隐地蛋白处理时会产生活性氧（AOS）。这种反应被中性粒细胞NADPH氧化酶抑制剂二亚苯基碘鎓阻断。当用针对人类小G蛋白Rac2的抗体探测烟草细胞的微粒体提取物时，在18.5 kDa和20.5 kDa处检测到两种免疫反应性蛋白。用烟草细胞的胞质提取物进行同样的实验，结果发现在仅经隐地蛋白处理的细胞中，在21.5 kDa处有一个强免疫反应性蛋白。这种胞质蛋白的出现与激发细胞产生AOS有关。这些结果为与中性粒细胞Rac2蛋白同源的小G蛋白可能参与植物激发子诱导的氧化爆发调节提供了证据。
