Ito Y, Kondo S, Chen G, Imanishi Y
PRESTO, JST, Kyoto, Japan.
FEBS Lett. 1997 Feb 17;403(2):159-62. doi: 10.1016/s0014-5793(97)00045-8.
Insulin was immobilized on prescribed areas of poly(ethylene terephthalate) film by photolithography. Chinese hamster ovary cells overexpressing human insulin receptors were cultured on the pattern-immobilized film. Pattern-immobilized insulin did not enhance cell adhesion but transduced a signal to the cells through phosphorylation of tyrosine residues of signal proteins. In addition, only cells on immobilized insulin grew in medium containing no serum. The enhancement of cell growth was considered to be a consequence of signal transduction. This microprocessing technique is useful for investigating the effect of immobilized biosignal molecules and for constructing new tissue engineering materials.
通过光刻技术将胰岛素固定在聚对苯二甲酸乙二酯薄膜的指定区域。在图案化固定的薄膜上培养过表达人胰岛素受体的中国仓鼠卵巢细胞。图案化固定的胰岛素不会增强细胞黏附，但会通过信号蛋白酪氨酸残基的磷酸化将信号传递给细胞。此外，只有固定有胰岛素的薄膜上的细胞能在无血清培养基中生长。细胞生长的增强被认为是信号转导的结果。这种微加工技术对于研究固定化生物信号分子的作用以及构建新型组织工程材料很有用。
