肝素的微图案固定化调节成纤维细胞生长因子促进细胞生长。
Micropattern-immobilization of heparin to regulate cell growth with fibroblast growth factor.
机构信息
Graduate School of Materials Science, NAIST, Ikoma, 630-0101, Japan.
出版信息
Cytotechnology. 2000 Jul;33(1-3):117-22. doi: 10.1023/A:1008154326954.
Abstract
Heparin was immobilized on a polystyrene plate in a specificpattern by photolithography. Heparin was coupled with azidoaniline. Thederivatized heparin was cast on the polystyrene plate from aqueoussolution. After drying, the plate was photo-irradiated in the presence of aphotomask. The micropatterning was confirmed by staining with a dye,ethydium bromide. Since heparin has negative charges, the cationic dyewas adsorbed on the regions where heparin was immobilized. In thepresence fibroblast growth factor (FGF), the growth of mouse fibroblastSTO cells was enhanced only on the heparin-immobilized regions. Thisresult indicated that micropattern-immobilized heparin activated FGF forcell growth activity.
摘要
肝素通过光刻技术以特定图案固定在聚苯乙烯板上。肝素与叠氮苯胺偶联。衍生化的肝素从水溶液中浇铸在聚苯乙烯板上。干燥后，在光掩模存在下进行光照射。通过用染料溴化乙锭染色来确认微图案。由于肝素带负电荷，因此阳离子染料吸附在肝素固定的区域上。在成纤维细胞生长因子（FGF）存在下，仅在肝素固定的区域，小鼠成纤维细胞 STO 细胞的生长得到增强。该结果表明，微图案固定的肝素激活了 FGF 的细胞生长活性。
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