通过DNA聚合酶α检测急性白血病中的细胞增殖。

Cell proliferation detected by DNA polymerase alpha in acute leukemias.

作者信息

Wakita A, Nitta M, Mitomo Y, Takahashi M, Tanaka M, Kaneda T

机构信息

Second Department of Internal Medicine, Nagoya City University Medical School, Japan.

出版信息

Cancer Detect Prev. 1997;21(1):55-61.

PMID:9043764

Abstract

Fifty-nine patients with acute leukemia were studied by flow cytometry using monoclonal antibody against DNA polymerase alpha. Since fresh and frozen cells showed identical flow cytometric histograms, 86 cryopreserved samples (39 from peripheral blood and 47 from bone marrow) were used in this study. The DNA polymerase alpha-positive population ranged from 16.3 to 84.7% in peripheral blood, and from 6.5 to 92.1% in bone marrow. A positive correlation (r = 0.80; p < 0.01) was found between DNA polymerase alpha-positive populations in peripheral blood and bone marrow from the same patient. This suggests that the DNA polymerase alpha-positive population in the bone marrow can be estimated from that in peripheral blood. A negative correlation was observed between the positive population in bone marrow samples and the time to reach a nadir (r = -0.58: p < 0.01), while a positive correlation was found between the tumor cell count in bone marrow and the DNA polymerase alpha-positive population (r = 0.64; p < 0.01) in patients who responded to chemotherapy.

摘要

采用抗DNA聚合酶α单克隆抗体，通过流式细胞术对59例急性白血病患者进行了研究。由于新鲜细胞和冷冻细胞呈现出相同的流式细胞术直方图，因此本研究使用了86份冷冻保存样本（39份来自外周血，47份来自骨髓）。外周血中DNA聚合酶α阳性细胞群比例为16.3%至84.7%，骨髓中为6.5%至92.1%。在同一患者的外周血和骨髓中，DNA聚合酶α阳性细胞群之间存在正相关（r = 0.80；p < 0.01）。这表明可根据外周血中的DNA聚合酶α阳性细胞群来估算骨髓中的该细胞群。在骨髓样本中的阳性细胞群与达到最低点的时间之间观察到负相关（r = -0.58：p < 0.01），而在对化疗有反应的患者中，骨髓中的肿瘤细胞计数与DNA聚合酶α阳性细胞群之间存在正相关（r = 0.64；p < 0.01）。