[酶联免疫吸附测定法在肺炎患者肺炎链球菌诊断中的应用价值]
[Usefulness of an ELISA for the diagnosis of Streptococcus pneumoniae in patients with pneumonia].
作者信息
Mata M, Sesma P, Bernárdez I
机构信息
Departamento de Microbiología, Facultad de Biología, Universidad de Santiago de Compostela.
出版信息
Enferm Infecc Microbiol Clin. 1996 Apr;14(4):255-7.
BACKGROUND
To evaluate the etiologic role of Streptococcus pneumoniae in a series of patients with community-acquired pneumonia.
METHODS
By an direct ELISA the presence of Streptococcus pneumoniae capsular polysaccharide antigen in serum and urine from 125 patients with community pneumonia was evaluated. Horseradish peroxidase-labeled polyvalent antiserum (Omniserum) was used. Working dilutions of reagents were determined by checkerboarding. Positive control antigen consisted of Pneumovax 23 and negative control consisted of serum and urine samples from healthy subjects.
RESULTS
Antigen detection was positive in 8 of 124 (6.45%; CI 95%: 2.80-12.30%) serum samples and in 15 of 115 (13.04%; CI 95%; 7.51-20.68%) urine samples concentrated 25-fold. Pneumococcal pneumonia was diagnosed by ELISA in 17 cases (13.60%; CI 95%: 8.15-20.95%), of which one had positive blood culture. The sensitivity of the ELISA in 24 patients with definite pneumococcal pneumonia diagnosed in this series was 70.83%.
CONCLUSION
These results suggest that the ELISA is a useful method in rapid diagnosis of Streptococcus pneumoniae in patients with pneumonia.
背景
评估肺炎链球菌在一系列社区获得性肺炎患者中的病因学作用。
方法
采用直接酶联免疫吸附测定法（ELISA）评估125例社区肺炎患者血清和尿液中肺炎链球菌荚膜多糖抗原的存在情况。使用辣根过氧化物酶标记的多价抗血清（全抗血清）。通过棋盘滴定法确定试剂的工作稀释度。阳性对照抗原由23价肺炎球菌多糖疫苗（Pneumovax 23）组成，阴性对照由健康受试者的血清和尿液样本组成。
结果
在124份血清样本中的8份（6.45%；95%置信区间：2.80 - 12.30%）以及25倍浓缩的115份尿液样本中的15份（13.04%；95%置信区间：7.51 - 20.68%）中抗原检测呈阳性。通过ELISA诊断出17例肺炎球菌肺炎（13.60%；95%置信区间：8.15 - 20.95%），其中1例血培养呈阳性。在本系列中确诊的24例明确的肺炎球菌肺炎患者中，ELISA的敏感性为70.83%。
结论
这些结果表明，ELISA是快速诊断肺炎患者肺炎链球菌感染的一种有用方法。
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