Kariagina I Iu, Slepysheva V V, Kozlov A V
Klin Lab Diagn. 1996 Nov-Dec(6):27-8.
A simple, economic, and available method for measuring protein in the urine is proposed. It is based on the capacity of bromophenol blue stain to form a complex with proteins in acid medium, which is characterized by absorption maximum at a wave-length of 597 nm. The proposed method is compared with the sulfosalicylic acid test and the Biuret method.
提出了一种简单、经济且可行的尿液蛋白质检测方法。该方法基于溴酚蓝染料在酸性介质中与蛋白质形成复合物的能力,其特征在于在597纳米波长处有最大吸收。将所提出的方法与磺基水杨酸试验和双缩脲法进行了比较。