Schosinsky K H, Vargas M, Luz Esquivel A, Chavarria M A
Clin Chem. 1987 Feb;33(2 Pt 1):223-6.
This procedure for routine quantification of albumin in urine is based on the dye-binding properties of albumin with bromphenol blue. The absorbance of 100 microL of urine mixed with 3 mL of color reagent is measured against blank reagent at 610 nm after 30 s. Results vary linearly with albumin concentration up to 6 g/L. The reaction is pH independent in the physiological range. It is not subject to substantial interference by uric acid, creatinine, calcium, sodium chloride, or bilirubin. The presence of globulins produces a small positive error. Within-run precision (CV) was 4.8, 1.5, and 0.9%, and day-to-day precision was 11.2, 2.0, and 1.9%, for samples containing albumin at about 0.1, 1.0, and 6.0 g/L, respectively. Results by a radial-immunodiffusion method (x) correlated well with those by the proposed method (y): r = 0.986; y = 0.98x + 0.096; n = 64. The method can also be used to detect globulins, such as Bence Jones protein, by measuring the ratio of the absorbance at 30 min to that at 30 s.
这种尿液中白蛋白常规定量方法基于白蛋白与溴酚蓝的染料结合特性。将100微升尿液与3毫升显色试剂混合，30秒后在610纳米处相对于空白试剂测量吸光度。结果在白蛋白浓度高达6克/升时与白蛋白浓度呈线性变化。该反应在生理范围内与pH无关。它不受尿酸、肌酐、钙、氯化钠或胆红素的实质性干扰。球蛋白的存在会产生小的正误差。对于白蛋白含量分别约为0.1、1.0和6.0克/升的样品，批内精密度（CV）分别为4.8%、1.5%和0.9%，日间精密度分别为11.2%、2.0%和1.9%。放射免疫扩散法（x）的结果与所提出方法（y）的结果相关性良好：r = 0.986；y = 0.98x + 0.096；n = 64。该方法还可通过测量30分钟时与30秒时的吸光度比值来检测球蛋白，如本-周蛋白。
