Marroni N P, Marroni C A, Esser T, Brugnara L, Casadevall M, Piqué J M
Departamento de Fisiología, Universidad Federal do Rio Grande do Sul.
Gastroenterol Hepatol. 1996 Dec;19(10):497-502.
The effects of chronic anemia on gastric damage induced by ethanol or aspirin have been investigated in rats. The role of free radicals and lipid peroxidation in that circumstance was also assessed. Chronic anemia was induced by replacement of 1.5 ml of blood by a plasma expander during 5 days. Under anesthesia, the stomach was perfused with 100% ethanol or acidified aspirin during 30 minutes. Thereafter, the rats were sacrificed, the stomachs removed and analyzed planimetrically for macroscopic damage. In addition, gastric tissue was collected and homogenized for assessment of toxic free radicals generation by chemoluminicense and lipid peroxidation by measuring reactive species of thiobarbituric acid (TBA-RS). Chronic anemia significantly protected against damage induced by ethanol or aspirin. The rate of toxic free radicals and the TBA-RS in the gastric mucosa was significantly reduced by anemia, either under ethanol or aspirin injury. It is concluded that anemia offers a general protection against gastric mucosal damage and that this protection is in part mediated by limitations on lipid peroxidation and toxic free radicals generation.
研究人员在大鼠中研究了慢性贫血对乙醇或阿司匹林所致胃损伤的影响。同时也评估了自由基和脂质过氧化在这种情况下的作用。通过在5天内用血浆扩容剂替代1.5毫升血液来诱导慢性贫血。在麻醉状态下，用100%乙醇或酸化阿司匹林对胃进行30分钟的灌注。此后，处死大鼠，取出胃并进行平面测量以分析宏观损伤。此外，收集胃组织并匀浆，通过化学发光法评估有毒自由基的产生，并通过测量硫代巴比妥酸反应性物质（TBA-RS）评估脂质过氧化。慢性贫血显著减轻了乙醇或阿司匹林所致的损伤。无论是在乙醇还是阿司匹林损伤下，贫血均显著降低了胃黏膜中有毒自由基的产生率和TBA-RS。研究得出结论，贫血对胃黏膜损伤具有普遍的保护作用，且这种保护部分是通过限制脂质过氧化和有毒自由基的产生来介导的。
