副溶血性弧菌钠/葡萄糖同向转运体（Sg1S）的一级结构与特性
Primary structure and properties of the Na+/glucose symporter (Sg1S) of Vibrio parahaemolyticus.
作者信息
Sarker R I, Ogawa W, Shimamoto T, Shimamoto T, Tsuchiya T
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
出版信息
J Bacteriol. 1997 Mar;179(5):1805-8. doi: 10.1128/jb.179.5.1805-1808.1997.
Abstract
Previously, we cloned and sequenced a DNA fragment from Vibrio parahaemolyticus and found four open reading frames (ORFs). Here, we clearly demonstrate that one of the ORFs, ORF1, is the gene (sglS) encoding a Na+/glucose symporter (SglS). We characterize the Na+/glucose symporter produced in Escherichia coli mutant (JM1100) cells which lack original glucose transport activity and galactose transport activity. We also show that phlorizin, a potent inhibitor of the SGLT1 Na+/glucose symporter of animal cells, inhibited glucose transport, but not galactose transport, via the SglS system.
摘要
此前，我们从副溶血性弧菌中克隆并测序了一个DNA片段，发现了四个开放阅读框（ORF）。在此，我们明确证明其中一个开放阅读框ORF1是编码Na⁺/葡萄糖同向转运体（SglS）的基因（sglS）。我们对在缺乏原始葡萄糖转运活性和半乳糖转运活性的大肠杆菌突变体（JM1100）细胞中产生的Na⁺/葡萄糖同向转运体进行了表征。我们还表明，根皮苷是动物细胞SGLT1 Na⁺/葡萄糖同向转运体的有效抑制剂，它通过SglS系统抑制葡萄糖转运，但不抑制半乳糖转运。
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