Dunn-Walters D K, Isaacson P G, Spencer J
UMDS St. Thomas' Campus, Department of Histopathology, London, GB.
Eur J Immunol. 1997 Feb;27(2):463-7. doi: 10.1002/eji.1830270217.
The origin of human ileal lamina propria (LP) plasma cells has been investigated using a technique based on microdissection of cells from immunohistochemically stained tissue sections. We have sequenced rearranged IgVH4.2 1, IgVH 5 family and IgVH6 genes from Peyer's patch germinal center (GC) cells and plasma cells from the ileal LP. Clonally related cells were identified by comparison of their complementarity determining region (CDR) 3 sequences and patterns of somatic mutation. We observed Ig genes from B cells in the GC of Peyer's patches which were related to Ig genes from plasma cells in the ileal LP, demonstrating that clonally related cells span these sites. In addition, groups of clonally related. but diversified plasma cells were common in the lamina propria. All Ig genes isolated from LP plasma cells were heavily mutated. The distribution of mutations in the CDR of the Ig heavy chain variable region (VH) genes which were considered to be the expressed alleles in LP plasma cells was consistent with an affinity-matured response. These observations provide compelling evidence for the origin of human ileal plasma cells from the Peyer's patches.
利用一种基于对免疫组织化学染色组织切片中的细胞进行显微切割的技术，对人回肠固有层（LP）浆细胞的起源进行了研究。我们对来自派尔集合淋巴结生发中心（GC）细胞以及回肠LP浆细胞的重排IgVH4.2 1、IgVH 5家族和IgVH6基因进行了测序。通过比较其互补决定区（CDR）3序列和体细胞突变模式来鉴定克隆相关细胞。我们观察到派尔集合淋巴结GC中的B细胞的Ig基因与回肠LP浆细胞的Ig基因相关，这表明克隆相关细胞跨越了这些部位。此外，克隆相关但多样化的浆细胞群在固有层中很常见。从LP浆细胞分离出的所有Ig基因都发生了高度突变。被认为是LP浆细胞中表达等位基因的Ig重链可变区（VH）基因的CDR中的突变分布与亲和力成熟反应一致。这些观察结果为人类回肠浆细胞起源于派尔集合淋巴结提供了令人信服的证据。
