CD44变体在分化型甲状腺癌中的表达增加。
Increased expression of CD44 variants in differentiated thyroid cancers.
作者信息
Takano T, Sumizaki H, Nakano K, Matsuzuka F, Kuma K, Amino N
机构信息
Department of Laboratory Medicine, Osaka University Medical School, Yamadaoka, Suita.
出版信息
Jpn J Cancer Res. 1996 Dec;87(12):1245-50. doi: 10.1111/j.1349-7006.1996.tb03139.x.
Abstract
Expression of CD44 variants in thyroid tumors was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) with a fluorescent image analyzer. Increased expression of CD44 variants compared with normal thyroid tissues was observed in most thyroid follicular tumors, especially in follicular carcinomas, poorly differentiated papillary carcinomas and some follicular adenomas. However, variants were hardly detectable in an anaplastic carcinoma. Analysis with restriction enzymes revealed that the major PCR product, consisting of variant bands, was derived from CD44E. Therefore, the expression of CD44E may be associated with the proliferation of differentiated thyroid cells.
摘要
采用荧光图像分析仪通过逆转录-聚合酶链反应（RT-PCR）分析甲状腺肿瘤中CD44变体的表达情况。在大多数甲状腺滤泡性肿瘤中，尤其是在滤泡癌、低分化乳头状癌和一些滤泡性腺瘤中，观察到与正常甲状腺组织相比CD44变体的表达增加。然而，在未分化癌中几乎检测不到变体。用限制性内切酶分析表明，由变带组成的主要PCR产物来源于CD44E。因此，CD44E的表达可能与分化型甲状腺细胞的增殖有关。
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