Cui Y, Lee T F, Westly J, Wang L C
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Brain Res. 1997 Feb 7;747(2):189-94. doi: 10.1016/s0006-8993(96)01092-x.
To localize and quantify the state-dependent changes in various opioid receptor subtypes in the limbic system of non-hibernating and hibernating Columbian ground squirrels, quantitative receptor-binding autoradiography was used. Compared to the non-hibernating animals, the binding density of [3H]-[D-Pen2,5]-enkephalin (DPDPE) to the delta receptor in the lateral septum, CA3, and the hippocampal fissure of the hippocampal formation was significantly decreased in the hibernating ground squirrels. A significant reduction in the binding density of [3H]-[D-Ala2,N-Me-Phe4,Gly-ol5]-enkephalin (DAGO) to mu receptor was also observed in the medial septum and the CA3 region of the hippocampus of the hibernating animals. In contrast, a decrease in [3H]ethylketocyclazocine (EKC) binding to the kappa receptor was only observed in the claustrum and CA3 of the hippocampus during hibernation. The differential changes in binding to various opioid receptors suggest that different opioid subtypes may exert different physiological roles in regulating the specific states (entrance, maintenance and arousal) of a hibernation bout.
为了定位和量化非冬眠和冬眠的哥伦比亚地松鼠边缘系统中各种阿片受体亚型的状态依赖性变化，采用了定量受体结合放射自显影术。与非冬眠动物相比，冬眠的地松鼠中，[3H]-[D-青霉胺2,5]-脑啡肽（DPDPE）与海马结构外侧隔、CA3和海马裂中δ受体的结合密度显著降低。在冬眠动物海马的内侧隔和CA3区域，也观察到[3H]-[D-丙氨酸2，N-甲基苯丙氨酸4，甘氨酸-ol5]-脑啡肽（DAGO）与μ受体的结合密度显著降低。相比之下，仅在冬眠期间在海马的屏状核和CA3中观察到[3H]乙基酮环唑新（EKC）与κ受体结合的减少。与各种阿片受体结合的差异变化表明，不同的阿片亚型在调节冬眠周期的特定状态（进入、维持和觉醒）中可能发挥不同的生理作用。
