甘蓝自交不亲和基因座在RFLP和RAPD图谱中的定位
Location of the self-incompatibility locus in an RFLP and RAPD map of Brassica oleracea.
作者信息
Camargo L E, Savides L, Jung G, Nienhuis J, Osborn T C
机构信息
Department of Agronomy, University of Wisconsin, Madison 53706, USA.
出版信息
J Hered. 1997 Jan-Feb;88(1):57-60. doi: 10.1093/oxfordjournals.jhered.a023057.
A single locus controlling self-incompatibility (SI) was located on an RFLP and RAPD linkage map of Brassica oleracea by assaying for SI phenotype and segregation of an RFLP locus detected by the cloned St gene SLG6. The map was developed using an F2 population derived from a cabbage x broccoli cross and included 112 RFLP and 47 RAPD loci arranged into nine main linkage groups covering 921 cM. A portion of the population was assayed for St reaction by pollinating with the broccoli parent. The SI reactions cosegregated precisely with the RFLP locus detected by SLG6, and this locus mapped to linkage group 2.
通过检测甘蓝型油菜的自交不亲和（SI）表型以及克隆的St基因SLG6检测到的一个RFLP位点的分离情况，在甘蓝的RFLP和RAPD连锁图谱上定位了一个控制自交不亲和的单一位点。该图谱是利用甘蓝型油菜与西兰花杂交产生的F2群体构建的，包含112个RFLP位点和47个RAPD位点，排列成9个主要连锁群，覆盖921厘摩。通过用西兰花亲本授粉，对部分群体进行了St反应检测。SI反应与SLG6检测到的RFLP位点精确共分离，该位点定位于连锁群2。
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