Khachaturov E N
Ontogenez. 1977;8(2):205-8.
It was shown that serious errors in DNA cytofluorimetry may be due to incorrect illumination of photocathode of the photomultiplier in the cytofluorimeters and non-observance of the requirements to the nuclear structures of the cells under measurement with respect to their chemical integrity and thickness. The methods of the optimal illumination of photocathode in the cytofluorimeters and the criteria of selection of the nuclear structures on slides are considered with the aim of obtaining the objective results of DNA cytofluorimetry.
结果表明,DNA细胞荧光测定法中的严重误差可能是由于细胞荧光计中光电倍增管的光电阴极照明不正确,以及在测量细胞的核结构时未遵守关于其化学完整性和厚度的要求所致。为了获得DNA细胞荧光测定法的客观结果,本文考虑了细胞荧光计中光电阴极的最佳照明方法以及载玻片上核结构的选择标准。