Mishima M
Second Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.
Arch Androl. 1997 Mar-Apr;38(2):113-26. doi: 10.3109/01485019708987888.
Human spermatozoa do not attach to the zona pellucida of other species because of their high specificity compared to that of other animals in recognizing the zona pellucida. This study describes the recognition mechanism of human spermatozoa through trypsin treatment of the ovum of human and other species. This research has revealed two findings: (1) The "lattice-like" structure, the surface of the zona pellucida of the hamster ovum, was lost and became a uniformly coarse and flat structure. More than 200 human spermatozoa attached to it. The number of attaching spermatozoa increased significantly along with the prolongation of the incubation period and in direct dependence on the spermatozoa concentration. (2) Human spermatozoa did not attach to the zona pellucida of mouse, rat, and human ovum. This result confirmed that human spermatozoa attach to hamster ovum if modified with trypsin.
与其他动物相比，人类精子在识别透明带方面具有高度特异性，因此不会附着于其他物种的透明带。本研究通过对人类及其他物种的卵子进行胰蛋白酶处理，描述了人类精子的识别机制。这项研究揭示了两个发现：（1）仓鼠卵子透明带表面的“格子状”结构消失，变成了均匀粗糙的扁平结构。200多个人类精子附着在上面。随着孵育时间的延长，附着的精子数量显著增加，且直接依赖于精子浓度。（2）人类精子不会附着于小鼠、大鼠和人类卵子的透明带。该结果证实，经胰蛋白酶处理后的人类精子会附着于仓鼠卵子。
