Murayama M, Yasuda H, Nishimura Y, Asahi M
Department of Dermatology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Arch Dermatol Res. 1997 Jan;289(2):98-103. doi: 10.1007/s004030050162.
We investigated the effects of the antibodies against the adhesion molecules leucocyte function associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) on mouse allergic contact hypersensitivity. Mice were injected intraperitoneally with both antibodies before sensitization by an epicutaneous application of dinitrofluorobenzene (DNFB). Simultaneous administration of the antibodies induced suppression of ear swelling in the effector phase of contact hypersensitivity. To show the effect of antibodies in vitro, lymph node cells (LNC) of mice treated with antibodies or with phosphate-buffered saline (PBS) were cultured in the presence of dinitrobenzene sulphonic sodium salts (DNBS), and the production of interleukin-2, interleukin-4 and interleukin-10 in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA). It was found that the production of interleukin-2 in the cells of mice treated with the antibodies was significantly lower than in the cells of PBS-treated mice. On the other hand, no difference was noted in the production of interleukin-4 or interleukin-10. Our results indicate that in vivo simultaneous administration of antibodies to cell adhesion molecules before hapten sensitization induces the suppression of contact hypersensitivity in mice and that the suppression may be due to the inhibition of the production of interleukin-2.
我们研究了抗黏附分子白细胞功能相关抗原-1（LFA-1）和细胞间黏附分子-1（ICAM-1）的抗体对小鼠过敏性接触超敏反应的影响。在用二硝基氟苯（DNFB）经皮致敏之前，给小鼠腹腔注射这两种抗体。同时给予抗体可在接触超敏反应的效应阶段诱导耳部肿胀的抑制。为了在体外显示抗体的作用，将用抗体或磷酸盐缓冲盐水（PBS）处理的小鼠的淋巴结细胞（LNC）在二硝基苯磺酸钠盐（DNBS）存在下培养，并使用酶联免疫吸附测定（ELISA）测量培养上清液中白细胞介素-2、白细胞介素-4和白细胞介素-10的产生。发现用抗体处理的小鼠细胞中白细胞介素-2的产生明显低于用PBS处理的小鼠细胞。另一方面，白细胞介素-4或白细胞介素-10的产生没有差异。我们的结果表明，在体内半抗原致敏之前同时给予细胞黏附分子抗体可诱导小鼠接触超敏反应的抑制，并且这种抑制可能是由于白细胞介素-2产生的抑制。
