Luschnig C, Bachmair A
Department of Cytology and Genetics, University of Vienna, Austria.
Biol Chem. 1997 Jan;378(1):39-46. doi: 10.1515/bchm.1997.378.1.39.
Expression of components of the yeast retrotransposon Ty1 in E. coli was used to study early steps of retrotransposition. We find that polypeptides encompassing the capsid-forming component of Ty1 can assemble into particles in the heterologous host. Ty RNA can be detected in particle fractions. RNA packaging depends on features in the 5' part of Ty RNA, because deletion of 5' proximal sequences leads to decreased packaging efficiency. Protein domains required for the RNA packaging process reside between amino acids 146 and 394 of the capsid protein. The data presented also indicate that several early steps in the Ty1 life cycle can occur in a cellular environment which differs from yeast cytoplasm, supporting the notion that these steps are independent of host factors.
利用酵母逆转录转座子Ty1的组件在大肠杆菌中的表达来研究逆转录转座的早期步骤。我们发现，包含Ty1衣壳形成组件的多肽能够在异源宿主中组装成颗粒。在颗粒组分中可检测到Ty RNA。RNA包装取决于Ty RNA 5'部分的特征，因为删除5'近端序列会导致包装效率降低。RNA包装过程所需的蛋白质结构域位于衣壳蛋白的第146至394个氨基酸之间。所呈现的数据还表明，Ty1生命周期中的几个早期步骤可以在不同于酵母细胞质的细胞环境中发生，这支持了这些步骤独立于宿主因子的观点。
