He H, Rinaldo C R, Morel P A
Department of Medicine, University of Pittsburgh, Pittsburgh Cancer Institute, PA 15213, USA.
J Gen Virol. 1995 Jul;76 ( Pt 7):1603-10. doi: 10.1099/0022-1317-76-7-1603.
Cell-mediated immunity plays an important role in the host response against human cytomegalovirus (HCMV) infection. For the development of HCMV subunit vaccines it is essential to identify which HCMV proteins can induce protective immune responses in humans. We have studied the T cell proliferative responses to five HCMV proteins (IE1, IE2, pp71, gpUL18 and gB). These five proteins were produced using the maltose-binding protein (MBP) fusion protein system. T cell proliferative responses in 23 seropositive and six seronegative individuals were evaluated. None of the six seronegative individuals showed significant responses to any of the proteins. Of the 23 seropositive individuals, five responded to all five proteins, 14 responded to between one and four proteins and four responded to none of the proteins. The most commonly recognized proteins were gB (17/23, 74%) and IE2 (16/23, 70%). pp71 and IE1 were recognized by 10 of 23 (43%) individuals. Nine of 22 (41%) individuals tested responded to gpUL18, providing evidence that this protein is produced during infection. Our data indicate that a subunit vaccine composed of gB alone may not be sufficient to induce protective immunity in all individuals. The combination of two or three proteins may be more efficient as a potential vaccine.
细胞介导的免疫在宿主对抗人巨细胞病毒（HCMV）感染的反应中起重要作用。对于HCMV亚单位疫苗的研发而言，确定哪些HCMV蛋白可在人体中诱导保护性免疫反应至关重要。我们研究了针对五种HCMV蛋白（IE1、IE2、pp71、gpUL18和gB）的T细胞增殖反应。这五种蛋白是使用麦芽糖结合蛋白（MBP）融合蛋白系统生产的。评估了23名血清阳性和6名血清阴性个体的T细胞增殖反应。6名血清阴性个体对任何一种蛋白均未表现出明显反应。在23名血清阳性个体中，5人对所有五种蛋白有反应，14人对一至四种蛋白有反应，4人对任何蛋白均无反应。最常被识别的蛋白是gB（17/23，74%）和IE2（16/23，70%）。23名个体中有10人（43%）识别pp71和IE1。22名接受检测的个体中有9人（41%）对gpUL18有反应，这证明该蛋白在感染期间产生。我们的数据表明，仅由gB组成的亚单位疫苗可能不足以在所有个体中诱导保护性免疫。两种或三种蛋白的组合作为潜在疫苗可能更有效。
