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The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo.

作者信息

Peters S A, Mesnard J M, Kooter I M, Verver J, Wellink J, van Kammen A

机构信息

Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.

出版信息

J Gen Virol. 1995 Jul;76 ( Pt 7):1807-13. doi: 10.1099/0022-1317-76-7-1807.

DOI:10.1099/0022-1317-76-7-1807
PMID:9049386
Abstract

Processing of the 112 kDa ('112K') protein encoded by cowpea mosaic virus RNA 1 was examined in cowpea mesophyll protoplasts using a transient expression system. Cleavage of the 112K protein occurred via two alternative pathways either into VPg and 110K (24K + 87K) or into 26K (VPg + 24K) and 87K proteins. The 26K protein can be further cleaved into VPg and 24K proteins. The results support a model in which the 112K protein functions as the precursor of VPg during initiation of replication.

摘要

相似文献

1
The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo.
J Gen Virol. 1995 Jul;76 ( Pt 7):1807-13. doi: 10.1099/0022-1317-76-7-1807.
2
Processing of VPg-containing polyproteins encoded by the B-RNA from cowpea mosaic virus.豇豆花叶病毒B-RNA编码的含VPg多聚蛋白的加工过程。
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Sequence upstream of the 24K protease enhances cleavage of the cowpea mosaic virus B RNA-encoded polyprotein at the junction between the 24K and 87K proteins.24K蛋白酶上游的序列增强了豇豆花叶病毒B RNA编码的多聚蛋白在24K和87K蛋白之间连接处的切割。
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