Draoui M, Bellincampi L, Hospital V, Cadel S, Foulon T, Prat A, Barré N, Reichert U, Melino G, Cohen P
Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, URA CNRS 1682, Université Pierre et Marie Curie, Paris, France.
J Neurooncol. 1997 Jan;31(1-2):99-106. doi: 10.1023/a:1005745717231.
Under retinoic acid exposure, the three SK-N-BE(2)-derived human neuroblastoma cell lines, BE(2)-NA, BE(2)-SA and BE(2)-M17 undergo mainly differentiation, apoptosis or continue to proliferate, respectively. We have used this model system to study the modulation of the transcriptional expression of putative processing enzymes, two novel metallopeptidases; i.e. N-arginine dibasic convertase (NRD convertase; EC 3.4,24,61) and an aminopeptidase-B after exposure of the cells either to retinoic acid or to synthetic retinoid analogs. The data indicate that the two respective enzymes are differently modulated in the various cell lines. Whereas aminopeptidase-B expression is enhanced in most cases, NRD convertase appears to undergo opposite regulation in proliferating versus differentiating neuroblastoma cells. It is concluded that both genes might contain retinoic acid regulatory elements (RARE) in their promoters.
在视黄酸作用下，三种源自SK-N-BE(2)的人神经母细胞瘤细胞系，即BE(2)-NA、BE(2)-SA和BE(2)-M17，分别主要经历分化、凋亡或继续增殖。我们利用这个模型系统来研究假定的加工酶（两种新型金属肽酶）转录表达的调控，即N-精氨酸二肽基转换酶（NRD转换酶；EC 3.4,24,61）和氨肽酶B，在细胞暴露于视黄酸或合成类视黄醇类似物后进行研究。数据表明，这两种酶在不同的细胞系中受到不同的调控。虽然在大多数情况下氨肽酶B的表达增强，但NRD转换酶在增殖性与分化性神经母细胞瘤细胞中似乎受到相反的调控。得出的结论是，这两个基因的启动子中可能都含有视黄酸调控元件（RARE）。
