Figlerowicz M, Nagy P D, Bujarski J J
Department of Biological Sciences, Northern Illinois University, DeKalb 60115, USA.
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):2073-8. doi: 10.1073/pnas.94.5.2073.
Brome mosaic bromovirus (BMV), a positive-stranded RNA virus, supports both homologous and nonhomologous RNA recombinations. Two BMV (temperature-sensitive) mutants with alterations in the 2a protein, the putative RNA polymerase component of the viral replicase, were tested for their ability to support both types of recombination. Here we report that one of these mutants with the Leu-486 substituted by Phe did not support nonhomologous recombination. Effect on homologous recombination was mainly on the location and precision of crossover events. The other 2a mutant with Asn-458 substituted by Asp did not negatively affect either type of recombination. Apparently, BMV RNA polymerase participates differently in the two types of recombination events.
雀麦花叶病毒（BMV）是一种正链RNA病毒，支持同源和非同源RNA重组。对两个在病毒复制酶的假定RNA聚合酶组分2a蛋白上发生改变的BMV（温度敏感型）突变体进行了测试，以检测它们支持两种重组类型的能力。在此我们报告，其中一个将Leu-486替换为Phe的突变体不支持非同源重组。对同源重组的影响主要体现在交叉事件的位置和准确性上。另一个将Asn-458替换为Asp的2a突变体对两种重组类型均无负面影响。显然，BMV RNA聚合酶在两种重组事件中的参与方式不同。
