Williams R A, Baak J P, Meijer G A, Charlton I G
St. Vincent's Department of Anatomic Pathology, Wangaratta Service, University of Melbourne, Australia.
Anal Quant Cytol Histol. 1997 Feb;19(1):19-29.
To evaluate DNA image cytometry (ICM) in tissue sections, human and rat liver was used as a model. These tissues have distinct DNA ploidy histograms of mainly diploid (human) and predominantly tetraploid (rat) plus octaploid nuclei.
Section thickness varied from 4 to 12 microns in rat liver and 4 to 10 microns in human liver, in steps of 2 microns. The measurements were made using a commercially available ICM system, strictly following a measurement protocol.
Four-micrometer-thick sections gave very broad histograms. Six-micrometer-thick sections gave better results, but with increasing DNA PLOIDY (> 4c), modal peaks were broad and increasingly shifted to the left. Sections of 8-10 microns gave linear results of the 2c, 4c and 8c peaks and also the narrowest peaks. The 12-micron sections of rat liver were more difficult to measure, and the results were not improved.
The 8-micron sections gave the best results and were easiest to measure. These results are promising, but since liver is a fairly simple model, further studies are required to elucidate whether DNA ploidy measurements can be performed reliably on tissue sections of human tumors.
为了评估组织切片中的DNA图像细胞计量术（ICM），以人和大鼠肝脏作为模型。这些组织具有不同的DNA倍体直方图，主要为二倍体（人）以及主要为四倍体（大鼠）加八倍体核。
大鼠肝脏切片厚度在4至12微米之间变化，人肝脏切片厚度在4至10微米之间变化，步长为2微米。使用市售的ICM系统进行测量，严格遵循测量方案。
4微米厚的切片给出的直方图非常宽。6微米厚的切片给出了更好的结果，但随着DNA倍性增加（>4c），众数峰变宽并越来越向左偏移。8至10微米厚的切片给出了2c、4c和8c峰的线性结果，并且峰也是最窄的。大鼠肝脏12微米厚的切片更难测量，结果也没有改善。
8微米厚的切片给出了最佳结果且最易于测量。这些结果很有前景，但由于肝脏是一个相当简单的模型，需要进一步研究以阐明是否可以在人类肿瘤组织切片上可靠地进行DNA倍性测量。
