Higueruelo S, Romero R
Nephrology Research Unit, Hospital Universitario Germans Trias i Pujol, Badalona, Spain.
Pharmacol Res. 1996 Sep-Oct;34(3-4):117-20. doi: 10.1006/phrs.1996.0074.
To compare the effects of pravastatin, a 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase inhibitor, on DNA synthesis in synchronized and proliferative rat glomerular mesangial cells (MCs), growth-arrested and proliferative MCs were exposed to medium containing 20% or 10% FBS, respectively, to induce mitogenesis. Different doses of pravastatin (10(-3)-10(-9) M) were added for 48 h. DNA synthesis was assessed by Bromodeoxyuridine (BrdU) incorporation. Pravastatin caused a significant (P < 0.05) dose-dependent reduction in BrdU incorporation in both serum-stimulated synchronized and proliferative MCs, but higher doses are required in the latter.
为比较3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶抑制剂普伐他汀对同步化和增殖性大鼠肾小球系膜细胞（MCs）DNA合成的影响，分别将生长停滞和增殖的MCs暴露于含20%或10%胎牛血清（FBS）的培养基中以诱导有丝分裂。加入不同剂量的普伐他汀（10⁻³ - 10⁻⁹ M）并作用48小时。通过溴脱氧尿苷（BrdU）掺入评估DNA合成。普伐他汀使血清刺激的同步化和增殖性MCs中的BrdU掺入均出现显著（P < 0.05）的剂量依赖性降低，但后者需要更高的剂量。
