Akov Y
Arch Virol. 1976;51(1-2):99-106. doi: 10.1007/BF01317838.
Acidification (at pH 5.75) of Semliki Forest virus and West Nile virus suspensions completely eliminated their hemagglutinating activity within several minutes, but did not affect their infectivity or change their ability to absorb homologous hemagglutination-inhibition antibodies. In order to assay antibody absorption it was necessary to remove all of the immune complex from the reaction mixture, because the immune complex inhibited additional hemagglutinin. Removal of the immune complex can be accomplished by the use of kaolin-absorbed virus. This procedure is simple and dependable and has been carried out with viruses from several groups--Toga-, Myxo-, Paramyxo- and Picornaviridae.
将塞姆利基森林病毒和西尼罗河病毒悬液酸化至pH 5.75，几分钟内即可完全消除其血凝活性，但不影响其感染性，也不会改变其吸附同源血凝抑制抗体的能力。为了检测抗体吸附情况，必须从反应混合物中去除所有免疫复合物，因为免疫复合物会抑制额外的血凝素。使用高岭土吸附病毒可去除免疫复合物。该方法简单可靠，已用于检测来自多个病毒科的病毒，如披膜病毒科、黏液病毒科、副黏液病毒科和小核糖核酸病毒科。
