Rast J P, Anderson M K, Strong S J, Luer C, Litman R T, Litman G W
Department of Pediatrics, University of South Florida, All Children's Hospital, St. Petersburg 33701, USA.
Immunity. 1997 Jan;6(1):1-11. doi: 10.1016/s1074-7613(00)80237-x.
A series of products were amplified using a PCR strategy based on short minimally degenerate primers and R. eglanteria (clearnose skate) spleen cDNA as template. These products were used as probes to select corresponding cDNAs from a spleen cDNA library. The cDNA sequences exhibit significant identity with prototypic (alpha, beta, gamma, and delta T cell antigen receptor (TCR) genes. Characterization of cDNAs reveals extensive variable region diversity, putative diversity segments, and varying degrees of junctional diversification. This demonstrates expression of both alpha/beta and gamma/delta TCR genes at an early level of vertebrate phylogeny and indicates that the three major known classes of rearranging antigen receptors were present in the common ancestor of the present-day jawed vertebrates.
使用基于短的最低限度简并引物和以皱鳃鲨脾脏cDNA为模板的PCR策略扩增了一系列产物。这些产物用作探针，从脾脏cDNA文库中筛选相应的cDNA。这些cDNA序列与原型（α、β、γ和δT细胞抗原受体（TCR）基因）具有显著的同一性。cDNA的特征分析揭示了广泛的可变区多样性、推定的多样性区段以及不同程度的连接多样性。这证明了α/β和γ/δTCR基因在脊椎动物系统发育的早期阶段都有表达，并表明当今有颌脊椎动物的共同祖先中存在三种主要的已知重排抗原受体类别。
