Quindós G, San Millan R, Bikandi J, Pontón J
Departamento de Inmunología, Microbiología y Parasi tologia, Facultad de Medicina y Odontología, Universidad del Pais Vasco, Bilbao.
Enferm Infecc Microbiol Clin. 1996 Dec;14(10):586-9.
Candida albicans infections are frequent in immunocompromised patients and a prompt diagnosis could favor an early and proper antifungal treatment. The rapid identification of clinical yeast isolates facilitate this diagnosis.
The utility of Fluoroplate Candida ready-to-use plates for Candida albicans rapid identification was evaluated with 653 clinical isolates from 23 yeast species, including 307 C. albicans plated onto Fluoroplate Candida agar (Merck, Germany). Rapid identification of C. albicans was based on the hydrolysis of 4-methylumbelliferyl-N-acetyl-beta-D-galactosaminide by the galactosaminidase activity of C. albicans producing white fluorescent colonies under ultraviolet light. Identification on Fluoroplate Candida was confirmed by germ tube, chlamydoconidia formation and API-ATB ID 32C assays.
Three hundred and five of 306 isolates showing fluorescent colonies were C. albicans and one was Candida glabrata (false positive). The rest of the isolates showed colonies without fluorescence and with the exception of two false negatives, these isolates were identified as non-C. albicans by other methods.
Fluoroplate Candida allows a rapid and excellent identification of C. albicans showing a sensitivity and specificity of 99.3 and 99.7%, respectively.
白色念珠菌感染在免疫功能低下患者中很常见，及时诊断有助于早期进行适当的抗真菌治疗。临床酵母菌分离株的快速鉴定有助于这一诊断。
使用Fluoroplate念珠菌即用型平板对来自23种酵母菌种的653株临床分离株进行白色念珠菌快速鉴定评估，其中包括307株接种在Fluoroplate念珠菌琼脂（德国默克公司）上的白色念珠菌。白色念珠菌的快速鉴定基于其半乳糖苷酶活性水解4-甲基伞形酮基-N-乙酰-β-D-氨基半乳糖苷，在紫外线下产生白色荧光菌落。通过芽管、厚垣孢子形成和API-ATB ID 32C检测确认在Fluoroplate念珠菌上的鉴定结果。
306株显示荧光菌落的分离株中有305株为白色念珠菌，1株为光滑念珠菌（假阳性）。其余分离株显示无荧光的菌落，除两例假阴性外，这些分离株通过其他方法鉴定为非白色念珠菌。
Fluoroplate念珠菌能够快速且出色地鉴定白色念珠菌，其敏感性和特异性分别为99.3%和99.7%。
