Ohno K, Kawasaki Y, Kubo T, Tohyama M
Department of Oto-rhino-laryngology, Osaka University Medical School, Japan.
Neuroscience. 1996 Mar;71(2):355-66. doi: 10.1016/0306-4522(95)00454-8.
We examined the distribution and ontogeny of two odorant-binding proteins in the rat at various stages of development from newborn to adult using northern blot and in situ hybridization methods. Our results demonstrated spatial segregation between odorant-binding protein and odorant-binding proteinII in nasal glandular tissues. Odorant-binding protein messenger RNA was expressed in the glandular system opening into the nasal vestibule, whereas odorant-binding proteinII messenger RNA was seen in the posterior glands of the nasal septum and in the vomeronasal glands. In addition, odorant-binding protein and odorant-binding proteinII messenger RNA levels increased during early postnatal stages with time courses that paralleled the anatomical development of the main olfactory system and the vomeronasal system, respectively. Our results suggest that odorant-binding proteinII functions as a pheromone transporter in the vomeronasal system.
我们使用Northern印迹法和原位杂交法，研究了从新生大鼠到成年大鼠不同发育阶段两种气味结合蛋白的分布和个体发生。我们的结果表明，在鼻腺组织中，气味结合蛋白和气味结合蛋白II存在空间分离。气味结合蛋白信使核糖核酸在通向鼻前庭的腺系统中表达，而气味结合蛋白II信使核糖核酸则见于鼻中隔后部腺体和犁鼻腺。此外，气味结合蛋白和气味结合蛋白II信使核糖核酸水平在出生后早期阶段升高，其时间进程分别与主嗅觉系统和犁鼻系统的解剖发育平行。我们的结果表明，气味结合蛋白II在犁鼻系统中作为一种信息素转运蛋白发挥作用。
