通过免疫电子显微镜对小鼠正中隆起中血管活性肠肽（VIP）进行细胞定位。
The cellular localization of vasoactive intestinal peptide (VIP) in the mouse median eminence by immuno-electron microscopy.
作者信息
Dalcik H, Chen I L, Dalcik C, Phelps C J
机构信息
Department of Hisotology and Embryology, Gulhane Military Medical Academy, Ankara, Turkey.
出版信息
Acta Histochem. 1996 Jan;98(1):53-9. doi: 10.1016/S0065-1281(96)80050-X.
The aim of this study was to examine, by use of pre-embedding immunocytochemistry, the ultrastructural localization of vasoactive intestinal peptide (VIP) immunoreactivity in the mouse median eminence. VIP immunoreactivity was observed in axonal profiles. The VIP-immunoreactive axonal profiles were in close proximity to non-immunoreactive axonal profiles that contained dense granular vesicles and clear vesicles and also to processes of tanycytes. VIP-immunoreactive terminals were observed in the proximity of the perivascular space and in the neuropil. Our results suggest that VIP-immunoreactive axon terminals may possibly interact with other non-immunoreactive axon terminals containing peptide and/or other transmitters at the level of the median eminence or may be released to the portal vasculature thereby to effect anterior pituitary cells.
本研究的目的是利用包埋前免疫细胞化学方法，检测小鼠正中隆起中血管活性肠肽（VIP）免疫反应性的超微结构定位。在轴突轮廓中观察到VIP免疫反应性。VIP免疫反应性轴突轮廓与含有致密颗粒小泡和清亮小泡的非免疫反应性轴突轮廓以及与伸长细胞的突起紧密相邻。在血管周围间隙附近和神经纤维网中观察到VIP免疫反应性终末。我们的结果表明，VIP免疫反应性轴突终末可能在正中隆起水平与其他含有肽和/或其他递质的非免疫反应性轴突终末相互作用，或者可能释放到门静脉血管系统，从而影响垂体前叶细胞。
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