Hawkins E C, Davidson M G, Meuten D J, Rottman J B, Kennedy-Stoskopf S, Levy J K, Ritchey J W, Cullins L D, Tompkins M B
Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
J Am Vet Med Assoc. 1997 Mar 1;210(5):648-50.
To determine whether it was possible to retrieve organisms, by means of bronchoalveolar lavage (BAL), from cats inoculated with Toxoplasma gondii.
Experimental study.
27 cats. Sixteen of the 27 were experimentally infected with feline immunodeficiency virus.
All cats were inoculated with T gondii tachyzoites. Cats were grouped on the basis of feline immunodeficiency virus status and route (IV or intra-arterial) and number of tachyzoites administered. Bronchoalveolar lavage was performed by means of a standard technique. Lavage fluid was evaluated cytologically for tachyzoites.
Clinical signs of toxoplasmosis varied widely among individual cats, but were generally most pronounced in group-1 and -2 cats (n = 5 each) and less pronounced in group-3 (n = 5) cats. Group-4 and -5 cats (n = 6 each) did not have clinical signs of toxoplasmosis. In 14 of the 15 cats in groups 1, 2, and 3, tachyzoites were detected in BAL fluid collected 7 days after inoculation. Tachyzoites were detected 14 days after inoculation in the single cat without tachyzoites 7 days after inoculation. A necropsy was performed on 9 of these cats, and tachyzoites were identified histologically in 4 of the 9. Tachyzoites were not detected in BAL fluid collected 3 days (n = 6) or 7 days (n = 6) after inoculation from the 12 cats in groups 4 and 5. Tachyzoites were not identified histologically in any of these 12 cats.
BAL may be useful in the diagnosis of toxoplasmosis, particularly in cats with signs of pulmonary involvement.
确定通过支气管肺泡灌洗（BAL）能否从接种刚地弓形虫的猫体内获取病原体。
实验研究。
27只猫。27只中的16只经实验感染猫免疫缺陷病毒。
所有猫均接种刚地弓形虫速殖子。根据猫免疫缺陷病毒感染状况、接种途径（静脉内或动脉内）和接种的速殖子数量对猫进行分组。采用标准技术进行支气管肺泡灌洗。对灌洗液进行细胞学检查以查找速殖子。
弓形虫病的临床症状在个体猫之间差异很大，但通常在第1组和第2组猫（每组n = 5）中最为明显，在第3组（n = 5）猫中则不太明显。第4组和第5组猫（每组n = 6）没有弓形虫病的临床症状。在接种后7天收集的第1、2和3组的15只猫中的14只的BAL液中检测到速殖子。在接种后7天未检测到速殖子的那只猫中，接种后14天检测到速殖子。对其中9只猫进行了尸检，9只中的4只在组织学上鉴定出速殖子。在第4组和第5组的12只猫接种后3天（n = 6）或7天（n = 6）收集的BAL液中未检测到速殖子。在这12只猫中的任何一只中均未在组织学上鉴定出速殖子。
BAL可能有助于弓形虫病的诊断，特别是对于有肺部受累迹象的猫。
