壬醛、反式-2-壬烯醛和4-羟基-2,3-反式-壬烯醛对兔血小板中花生四烯酸的环氧化酶和12-脂氧合酶代谢的影响。
Effects of nonanal, trans-2-nonenal and 4-hydroxy-2,3-trans-nonenal on cyclooxygenase and 12-lipoxygenase metabolism of arachidonic acid in rabbit platelets.
作者信息
Sakuma S, Fujimoto Y, Tagano S, Tsunomori M, Nishida H, Fujita T
机构信息
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
出版信息
J Pharm Pharmacol. 1997 Feb;49(2):150-3. doi: 10.1111/j.2042-7158.1997.tb06770.x.
The effects of nonanal, trans-2-nonenal and 4-hydroxy-2,3-trans-nonenal on the formation of thromboxane B2 (TXB2), 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) from exogenous arachidonic acid in washed rabbit platelets were examined. Nonanal and trans-2-nonenal at concentrations ranging from 0.25 to 2 microM inhibited TXB2, HHT and 12-HETE formation, reducing the amounts of these three arachidonic acid metabolites by 50% at nonanal and trans-2-nonenal concentrations of approximately 0.25 microM. The inhibition of TXB2, HHT and 12-HETE formation induced by 4-hydroxy-2,3-trans-nonenal (50% inhibition by 4-hydroxy-2,3-trans-nonenal at a concentration of approximately 100 microM) was 400 times weaker than that induced by nonanal and trans-2-nonenal. These results suggest that nonanal and trans-2-nonenal can be modulators of platelet arachidonic acid metabolism by affecting the activity of cyclooxygenase and 12-lipoxygenase.
研究了壬醛、反-2-壬烯醛和4-羟基-2,3-反-壬烯醛对洗涤过的兔血小板中由外源性花生四烯酸形成血栓素B2（TXB2）、12-羟基-5,8,10-十七碳三烯酸（HHT）和12-羟基-5,8,10,14-二十碳四烯酸（12-HETE）的影响。浓度范围为0.25至2 microM的壬醛和反-2-壬烯醛抑制TXB2、HHT和12-HETE的形成，在壬醛和反-2-壬烯醛浓度约为0.25 microM时，这三种花生四烯酸代谢物的量减少了50%。4-羟基-2,3-反-壬烯醛（在浓度约为100 microM时4-羟基-2,3-反-壬烯醛的抑制率为50%）对TXB2、HHT和12-HETE形成的抑制作用比壬醛和反-2-壬烯醛诱导的抑制作用弱400倍。这些结果表明，壬醛和反-2-壬烯醛可通过影响环氧化酶和12-脂氧合酶的活性来调节血小板花生四烯酸代谢。
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